Seiei Biotech Research Institute Co., Ltd. is a Japan-based biotechnology R&D enterprise specializing in the development of nitrogen removal technologies and integrated system solutions for wastewater treatment.
we boast over 15 years of expertise in enzyme engineering and microbial formulation development. Our mission is to combine microecological regulation, energy-efficient carbon reduction, and system integration to deliver innovative services to the global environmental market.
Let the light in.
Let the system awaken.
Let change begin — quietly
Innovative Low-Carbon Denitrification Technology: WaterBio-Max + AMX Synergistic Denitrification System Enhancer is a biotechnology-based solution applicable to A²/O and AO processes.
Note: AMX (Anammox bacteria) are formally known as anaerobic ammonium-oxidizing bacteria. They are highly efficient autotrophic denitrifying microorganisms that can convert ammonium (NH₄⁺) and nitrite (NO₂⁻) directly into nitrogen gas (N₂) without the need for oxygen.
This bacterial group was discovered in the late 1990s and is currently attracting global attention as a next-generation energy-saving denitrificat
| Item | WaterBio-Max + AMX Synergistic Denitrification Booster | Traditional Anammox Process |
|---|---|---|
| Reaction Type | Heterotrophic–autotrophic synergistic reaction | Autotrophic-dominated reaction |
| Startup Time | 3–7 days (depends on native microbial base) | 30–90 days (requires AMX seed sludge) |
| System Compatibility | Compatible with various conventional denitrification systems | Requires dedicated Anammox reactor |
| Low-Temperature Performance | Stable operation even under low temperatures (≤10℃) | Unstable under low-temperature conditions |
| Carbon Source Demand | Saves over 40% of external carbon source | Heavily depends on NO₂⁻ as electron donor |
| Dosing Method | Regular dosing of liquid formulation | Requires high-concentration AMX sludge inoculation |
| Load Tolerance | Capable of handling medium to high ammonia loads | Performance decreases under high loads |
| Maintenance Requirements | Stable operation without complex controls | Requires DO, pH, and NO₂⁻ control |
| Investment Cost | Nearly zero (direct dosing into existing systems) | Requires new reactor construction; high capital cost |
| Risk Management | Rapid recovery after system shock | Collapse leads to long-term recovery cycle |
No Retrofit. No Disturbance. Rock-Solid Stability.
Seamlessly integrates with conventional wastewater treatment processes like A²/O, AO, activating nitrogen removal in 3-7 days without altering equipment or disrupting operations, ensuring unwavering system reliability.
40-60% Carbon Reduction. Energy-Saving Excellence.
Leverages heterotrophic-autotrophic synergy to enhance NO₂⁻ accumulation and AMX metabolism, slashing external carbon dosing and aeration costs, ideal for low C/N ratios, cold climates, and high nitrogen loads, pioneering a new era of eco-efficiency.
Microbial Mastery. Enzyme-Powered Breakthrough.
Combines microecological regulation with enzyme activation to unlock a revolutionary nitrogen removal pathway, reengineering legacy systems without new reactors, delivering subtle intervention with profound transformation in wastewater treatment innovation.
Science-based validation technology
Through pilot studies conducted at multiple wastewater treatment plants and collaboration with universities and research institutes, the treatment performance has been scientifically validated. The results are visualized with graphs and data, providing reliability that ensures confidence in its implementation.
As climate change and ecosystem transformation become pressing global issues, the challenges of water treatment are no longer confined to a single country or region.
They are global, systemic, and directly linked to building a sustainable future.
Drawing on over 15 years of expertise in enzyme engineering and microbial agent development, we have developed an innovative denitrification enhancement technology— the WaterBio-Max + AMX Synergistic Denitrification System. This solution redefines next-generation nitrogen removal technologies.
We revive dormant treatment capacity without modifying equipment. We reduce carbon load without increasing energy input. We renew wastewater systems without halting operations.
This is more than a technical innovation— it is a philosophy rooted in achieving maximum transformation with minimal intervention. It honors existing infrastructure, while empowering it to exceed its limitations. We take pride in pioneering a new standard in wastewater management, and we are committed to leading the future through science and building a society in harmony with the environment.
We take pride in pioneering a new standard in wastewater management, and we are committed to leading the future through science and building a society in harmony with the environment.
To our partners, researchers, and future collaborators— let us reshape the future of water, together
Seiei Biotech Research Institute Co., Ltd.
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